Drug export and nutrient import: Insights from the bacterial model organisms Erwinia amylovora and Pseudomonas aeruginosa

  • The emergence of multidrug resistant bacteria is a serious medical and economical problem with global impact. Bacteria have evolved energy-dependent multidrug efflux pumps to prevent intracellular accumulation of toxic compounds and to survive in different ecological niches. On the other hand, the uptake of essential nutrients, while at the same time preventing the internalization of toxic molecules, are accompanying circumstances that cause an enormous selection pressure on bacterial populations. In the first part of this thesis, three resistance-nodulation-cell division (RND)-type multidrug efflux pumps, AcrD, MdtABC, and MdtUVW, from the plant pathogen Erwinia amylovora were characterized in terms of their substrate specificity, induction upon exposure to various stressors, and pathogenesis on apple and pear. Results of this study show that AcrD is an efflux pump conferring resistance to a limited number of amphiphilic compounds. MdtABC and MdtUVW were found to contribute to disease development in apple rootstocks. Moreover, we identified global and local regulators in E. amylovora and found that the BaeSR two-component system is involved in the activation of the RND-type efflux pumps AcrD and MdtABC. In the second part, the dipeptide uptake ABC transporter, DppBCDF, and its associated substrate-binding proteins, DppA1-A5, from Pseudomonas aeruginosa were characterized by a high-throughput method. We were able to determine the substrate specificity of this uptake transporter using the Biolog phenotype MicroArray technology.

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Publishing Institution:IRC-Library, Information Resource Center der Jacobs University Bremen
Granting Institution:Jacobs Univ.
Author:Daniel Pletzer
Referee:Helge Weingart, Georgi Muskhelishvili, Thilo Köhler
Advisor:Helge Weingart
Persistent Identifier (URN):urn:nbn:de:gbv:579-opus-1004562
Document Type:PhD Thesis
Date of Successful Oral Defense:2014/05/30
Year of Completion:2014
Date of First Publication:2014/08/01
Full Text Embargo Until:2014/08/31
PhD Degree:Biochemistry
School:SES School of Engineering and Science
Library of Congress Classification:Q Science / QR Microbiology / QR75-99.5 Bacteria / QR82 Systematic divisions. By family or higher taxa / QR82.E6 Enterobacteriaceae
Call No:Thesis 2014/17

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