Flow Cytometry Based Screening System for Finding and Improving Bioindustrial Important Biocatalysts - Cytochrome P450 BM3

  • Directed evolution presents one of the most common ways of tailoring biocatalysts for a certain application, especially when the information about the structure of biocatalyst is lacking. It consists of iterative cycles of diversity generation, on genetic level, and screening for the target property using a specific screening system. One of the bottlenecks of directed evolution experiment is the throughput of currently available screening systems. Recently, a new technology based on double emulsions and flow cytometry/FACS enabled screening for the enzyme activity with ultra high throughput (10[to the power]9 clones per round of directed evolution). In this work, we have optimized this technology for directed evolution of bacterial enzyme, Cytochrome P450 BM3.

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Publishing Institution:IRC-Library, Information Resource Center der Jacobs University Bremen
Granting Institution:Jacobs Univ.
Author:Milan Blanusa
Referee:Ulrich Schwaneberg, Sebastian Springer, Harald Gröger
Advisor:Ulrich Schwaneberg
Persistent Identifier (URN):urn:nbn:de:101:1-201305237178
Document Type:PhD Thesis
Language:English
Date of Successful Oral Defense:2009/12/04
Date of First Publication:2010/10/28
PhD Degree:Biochemical Engineering
School:SES School of Engineering and Science
Library of Congress Classification:T Technology / TP Chemical technology / TP155-156 Chemical engineering / TP156 Special processes and operations / TP156.S3 Screening
Call No:Thesis 2009/57

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